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Product Info Summary
| SKU: | M33947 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat, Yeast |
| Host: | Mouse |
| Application: | IP, IF, WB |
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Product info
Product Name
Anti-Histone H3 Monoclonal Antibody
View all Creatine kinase MT 1A Antibodies
SKU/Catalog Number
M33947
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Histone H3 Monoclonal Antibody catalog # M33947. Tested in IP, IF, WB applications. This antibody reacts with Human, Mouse, Rat, Yeast.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Histone H3 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M33947)
Host
Mouse
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
1G1
Isotype
IgG
Immunogen
Recombinant Protein
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M33947 is reactive to CKMT1A in Human, Mouse, Rat, Yeast
Applications
M33947 is guaranteed for IP, IF, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
47.037kDa
Background of Creatine kinase MT 1A
BIN-3 encodes an evolutionarily conserved and ubiquitously expressed member of the BAR superfamily of curved membrane and GTPase-binding proteins implicated in signal transduction and vesicular trafficking. In humans, Bin3 maps to chromosome 8p21.3, a region widely implicated in cancer suppression that is often deleted in non–Hodgkin’s lymphomas and various epithelial tumors. Study in mouse suggests that homozygous inactivation of Bin3 causes cataracts and an increased susceptibility to lymphomas during aging.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:2000-5000
IF1:100-500
IP 1:200
Validation Images & Assay Conditions
Click image to see more details
Figure 22. Immunofluorescent staining data of HIST1H3AHIST1H3BHIST1H3CHIST1H3DHIST1H3EHIST1H3FHIST1H3GHIST1H3HHIST1H3IHIST1H3J using Anti-Histone H3 HIST1H3AHIST1H3BHIST1H3CHIST1H3DHIST1H3EHIST1H3FHIST1H3GHIST1H3HHIST1H3IHIST1H3J Monoclonal Antibody (M33947).
Immunofluorescence (IF) analysis of HeLa
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Figure 10. Immunohistochemistry validation of HIST1H3AHIST1H3BHIST1H3CHIST1H3DHIST1H3EHIST1H3FHIST1H3GHIST1H3HHIST1H3IHIST1H3J using Anti-Histone H3 HIST1H3AHIST1H3BHIST1H3CHIST1H3DHIST1H3EHIST1H3FHIST1H3GHIST1H3HHIST1H3IHIST1H3J Monoclonal Antibody (M33947).
Immunohistochemical analysis of human uterus cancer tissue. M33947 was diluted at 1:200 (4°C
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Figure 11. Immunohistochemistry validation of HIST1H3AHIST1H3BHIST1H3CHIST1H3DHIST1H3EHIST1H3FHIST1H3GHIST1H3HHIST1H3IHIST1H3J using Anti-Histone H3 HIST1H3AHIST1H3BHIST1H3CHIST1H3DHIST1H3EHIST1H3FHIST1H3GHIST1H3HHIST1H3IHIST1H3J Monoclonal Antibody (M33947).
Immunohistochemical analysis of human uterus tissue. M33947 was diluted at 1:200 (4°C
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Figure 12. Immunohistochemistry validation of HIST1H3AHIST1H3BHIST1H3CHIST1H3DHIST1H3EHIST1H3FHIST1H3GHIST1H3HHIST1H3IHIST1H3J using Anti-Histone H3 HIST1H3AHIST1H3BHIST1H3CHIST1H3DHIST1H3EHIST1H3FHIST1H3GHIST1H3HHIST1H3IHIST1H3J Monoclonal Antibody (M33947).
Immunohistochemical analysis of mouse brain tissue. M33947 was diluted at 1:200 (4°C
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Figure 13. Immunohistochemistry validation of HIST1H3AHIST1H3BHIST1H3CHIST1H3DHIST1H3EHIST1H3FHIST1H3GHIST1H3HHIST1H3IHIST1H3J using Anti-Histone H3 HIST1H3AHIST1H3BHIST1H3CHIST1H3DHIST1H3EHIST1H3FHIST1H3GHIST1H3HHIST1H3IHIST1H3J Monoclonal Antibody (M33947).
Immunohistochemical analysis of mouse colon tissue. M33947 was diluted at 1:200 (4°C
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Figure 14. Immunohistochemistry validation of HIST1H3AHIST1H3BHIST1H3CHIST1H3DHIST1H3EHIST1H3FHIST1H3GHIST1H3HHIST1H3IHIST1H3J using Anti-Histone H3 HIST1H3AHIST1H3BHIST1H3CHIST1H3DHIST1H3EHIST1H3FHIST1H3GHIST1H3HHIST1H3IHIST1H3J Monoclonal Antibody (M33947).
Immunohistochemical analysis of mouse kidney tissue. M33947 was diluted at 1:200 (4°C
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Figure 15. Immunohistochemistry validation of HIST1H3AHIST1H3BHIST1H3CHIST1H3DHIST1H3EHIST1H3FHIST1H3GHIST1H3HHIST1H3IHIST1H3J using Anti-Histone H3 HIST1H3AHIST1H3BHIST1H3CHIST1H3DHIST1H3EHIST1H3FHIST1H3GHIST1H3HHIST1H3IHIST1H3J Monoclonal Antibody (M33947).
Immunohistochemical analysis of mouse liver tissue. M33947 was diluted at 1:200 (4°C
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Figure 16. Immunohistochemistry validation of HIST1H3AHIST1H3BHIST1H3CHIST1H3DHIST1H3EHIST1H3FHIST1H3GHIST1H3HHIST1H3IHIST1H3J using Anti-Histone H3 HIST1H3AHIST1H3BHIST1H3CHIST1H3DHIST1H3EHIST1H3FHIST1H3GHIST1H3HHIST1H3IHIST1H3J Monoclonal Antibody (M33947).
Immunohistochemical analysis of mouse testis tissue. M33947 was diluted at 1:200 (4°C
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Figure 17. Immunohistochemistry validation of HIST1H3AHIST1H3BHIST1H3CHIST1H3DHIST1H3EHIST1H3FHIST1H3GHIST1H3HHIST1H3IHIST1H3J using Anti-Histone H3 HIST1H3AHIST1H3BHIST1H3CHIST1H3DHIST1H3EHIST1H3FHIST1H3GHIST1H3HHIST1H3IHIST1H3J Monoclonal Antibody (M33947).
Immunohistochemical analysis of rat brain tissue. Anti-Cleaved-Caspase-1 (D210) antibody was diluted at 1:200 (4°C
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Figure 18. Immunohistochemistry validation of HIST1H3AHIST1H3BHIST1H3CHIST1H3DHIST1H3EHIST1H3FHIST1H3GHIST1H3HHIST1H3IHIST1H3J using Anti-Histone H3 HIST1H3AHIST1H3BHIST1H3CHIST1H3DHIST1H3EHIST1H3FHIST1H3GHIST1H3HHIST1H3IHIST1H3J Monoclonal Antibody (M33947).
Immunohistochemical analysis of rat kidney tissue. Anti-Cleaved-Caspase-1 (D210) antibody was diluted at 1:200 (4°C
Click image to see more details
Figure 19. Immunohistochemistry validation of HIST1H3AHIST1H3BHIST1H3CHIST1H3DHIST1H3EHIST1H3FHIST1H3GHIST1H3HHIST1H3IHIST1H3J using Anti-Histone H3 HIST1H3AHIST1H3BHIST1H3CHIST1H3DHIST1H3EHIST1H3FHIST1H3GHIST1H3HHIST1H3IHIST1H3J Monoclonal Antibody (M33947).
Immunohistochemical analysis of rat spinal cord tissue. Anti-Cleaved-Caspase-1 (D210) antibody was diluted at 1:200 (4°C
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Figure 2. Immunohistochemistry validation of HIST1H3AHIST1H3BHIST1H3CHIST1H3DHIST1H3EHIST1H3FHIST1H3GHIST1H3HHIST1H3IHIST1H3J using Anti-Histone H3 HIST1H3AHIST1H3BHIST1H3CHIST1H3DHIST1H3EHIST1H3FHIST1H3GHIST1H3HHIST1H3IHIST1H3J Monoclonal Antibody (M33947).
Immunohistochemical analysis of human-appendix tissue. Anti-Histone H3 at 1:200 (4°C
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Figure 20. Immunohistochemistry validation of HIST1H3AHIST1H3BHIST1H3CHIST1H3DHIST1H3EHIST1H3FHIST1H3GHIST1H3HHIST1H3IHIST1H3J using Anti-Histone H3 HIST1H3AHIST1H3BHIST1H3CHIST1H3DHIST1H3EHIST1H3FHIST1H3GHIST1H3HHIST1H3IHIST1H3J Monoclonal Antibody (M33947).
Immunohistochemical analysis of rat spleen tissue. Anti-Cleaved-Caspase-1 (D210) antibody was diluted at 1:200 (4°C
Click image to see more details
Figure 21. Immunohistochemistry validation of HIST1H3AHIST1H3BHIST1H3CHIST1H3DHIST1H3EHIST1H3FHIST1H3GHIST1H3HHIST1H3IHIST1H3J using Anti-Histone H3 HIST1H3AHIST1H3BHIST1H3CHIST1H3DHIST1H3EHIST1H3FHIST1H3GHIST1H3HHIST1H3IHIST1H3J Monoclonal Antibody (M33947).
Immunohistochemical analysis of rat testis tissue. Anti-Cleaved-Caspase-1 (D210) antibody was diluted at 1:200 (4°C
Click image to see more details
Figure 3. Immunohistochemistry validation of HIST1H3AHIST1H3BHIST1H3CHIST1H3DHIST1H3EHIST1H3FHIST1H3GHIST1H3HHIST1H3IHIST1H3J using Anti-Histone H3 HIST1H3AHIST1H3BHIST1H3CHIST1H3DHIST1H3EHIST1H3FHIST1H3GHIST1H3HHIST1H3IHIST1H3J Monoclonal Antibody (M33947).
Immunohistochemical analysis of human colon tissue. Anti-Histone H3 at 1:200 (4°C
Click image to see more details
Figure 4. Immunohistochemistry validation of HIST1H3AHIST1H3BHIST1H3CHIST1H3DHIST1H3EHIST1H3FHIST1H3GHIST1H3HHIST1H3IHIST1H3J using Anti-Histone H3 HIST1H3AHIST1H3BHIST1H3CHIST1H3DHIST1H3EHIST1H3FHIST1H3GHIST1H3HHIST1H3IHIST1H3J Monoclonal Antibody (M33947).
Immunohistochemical analysis of human liver cancer tissue. Anti-Histone H3 at 1:200 (4°C
Click image to see more details
Figure 5. Immunohistochemistry validation of HIST1H3AHIST1H3BHIST1H3CHIST1H3DHIST1H3EHIST1H3FHIST1H3GHIST1H3HHIST1H3IHIST1H3J using Anti-Histone H3 HIST1H3AHIST1H3BHIST1H3CHIST1H3DHIST1H3EHIST1H3FHIST1H3GHIST1H3HHIST1H3IHIST1H3J Monoclonal Antibody (M33947).
Immunohistochemical analysis of human lung cancer tissue. Anti-Histone H3 at 1:200 (4°C
Click image to see more details
Figure 6. Immunohistochemistry validation of HIST1H3AHIST1H3BHIST1H3CHIST1H3DHIST1H3EHIST1H3FHIST1H3GHIST1H3HHIST1H3IHIST1H3J using Anti-Histone H3 HIST1H3AHIST1H3BHIST1H3CHIST1H3DHIST1H3EHIST1H3FHIST1H3GHIST1H3HHIST1H3IHIST1H3J Monoclonal Antibody (M33947).
Immunohistochemical analysis of human lung tissue. Anti-Histone H3 at 1:200 (4°C
Click image to see more details
Figure 7. Immunohistochemistry validation of HIST1H3AHIST1H3BHIST1H3CHIST1H3DHIST1H3EHIST1H3FHIST1H3GHIST1H3HHIST1H3IHIST1H3J using Anti-Histone H3 HIST1H3AHIST1H3BHIST1H3CHIST1H3DHIST1H3EHIST1H3FHIST1H3GHIST1H3HHIST1H3IHIST1H3J Monoclonal Antibody (M33947).
Immunohistochemical analysis of human stomach cancer tissue. Anti-Histone H3 at 1:200 (4°C
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Figure 8. Immunohistochemistry validation of HIST1H3AHIST1H3BHIST1H3CHIST1H3DHIST1H3EHIST1H3FHIST1H3GHIST1H3HHIST1H3IHIST1H3J using Anti-Histone H3 HIST1H3AHIST1H3BHIST1H3CHIST1H3DHIST1H3EHIST1H3FHIST1H3GHIST1H3HHIST1H3IHIST1H3J Monoclonal Antibody (M33947).
Immunohistochemical analysis of human stomach tissue. M33947 was diluted at 1:200 (4°C
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Figure 9. Immunohistochemistry validation of HIST1H3AHIST1H3BHIST1H3CHIST1H3DHIST1H3EHIST1H3FHIST1H3GHIST1H3HHIST1H3IHIST1H3J using Anti-Histone H3 HIST1H3AHIST1H3BHIST1H3CHIST1H3DHIST1H3EHIST1H3FHIST1H3GHIST1H3HHIST1H3IHIST1H3J Monoclonal Antibody (M33947).
Immunohistochemical analysis of human tonsil tissue. M33947 was diluted at 1:200 (4°C
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Figure 23. IP validation of HIST1H3AHIST1H3BHIST1H3CHIST1H3DHIST1H3EHIST1H3FHIST1H3GHIST1H3HHIST1H3IHIST1H3J using Anti-Histone H3 HIST1H3AHIST1H3BHIST1H3CHIST1H3DHIST1H3EHIST1H3FHIST1H3GHIST1H3HHIST1H3IHIST1H3J Monoclonal Antibody (M33947).
Immunoprecipitation (IP) analysis: 1) Input: HeLa Cell Lysate.
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Figure 1. Western blotting validation for Anti-Histone H3 HIST1H3AHIST1H3BHIST1H3CHIST1H3DHIST1H3EHIST1H3FHIST1H3GHIST1H3HHIST1H3IHIST1H3J Monoclonal Antibody M33947
Western blot (WB) analysis of Histone H3 monoclonal antibody.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For CKMT1A (Source: Uniprot.org, NCBI)
Gene Name
CKMT1A
Full Name
Creatine kinase U-type, mitochondrial
Weight
47.037kDa
Superfamily
ATP:guanido phosphotransferase family
Alternative Names
Acidic-Type Mitochondrial Creatine Kinase; CKMT; CKMT1; CKMT1A; Creatine Kinase U-Type, Mitochondrial; Creatine Kinase, Mitochondrial 1 (Ubiquitous); Creatine Kinase, Mitochondrial 1A; EC 2.7.3.2; Mia-CK; Ubiquitous Mitochondrial Creatine Kinase; U-MtCK CKMT1A CKMT1, U-MtCK, mia-CK creatine kinase, mitochondrial 1A creatine kinase U-type, mitochondrial|acidic-type mitochondrial creatine kinase|creatine kinase, mitochondrial 1 (ubiquitous)|ubiquitous mitochondrial creatine kinase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CKMT1A, check out the CKMT1A Infographic
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In this infographic, you will see the following information for CKMT1A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Histone H3 Monoclonal Antibody (M33947)
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